Chrysin (5,7-dihydroxyflavone) is a flavone found in high concentrations in several plant extracts, honey, and propolis that has been shown to have anticancer activity. However, due to its low solubility and bioavailability, it necessitates using a delivery system to achieve its therapeutic goals. In this study, chrysin loaded on gold nanoparticles (CHR-AuNPs) was prepared by chemical synthesis. The current biosynthesis method is simple and low cost. The formation of chrysin loaded onto gold nanoparticles (CHR-AuNPs) was observed after the preparation of gold nanoparticles and the addition of chrysin (dissolved pale yellow) overnight by changing the color of the prepared solution from red to light violet. Chemical synthesis was used to generate chrysin-loaded gold nanoparticles (CHR-AuNPs) identified by UV, FTIR, XRD, EDX, zeta and FESEM potential analysis. The absorption spectra of chrysin on gold nanoparticles showed a curved absorption of 550 nm. FTIR was used to evaluate the molecular and functional groups of chrysin, which were loaded onto gold nanoparticles. XRD and FESEM studies confirmed the presence of chrysin loaded on gold nanotechnology particles, and regular spherical particles were found with an average size of 30-50 nm. The antioxidant activity of CHR-AuNPs was tested against DPPH. The results revealed that CHR-AuNPs had a strong antioxidant effect and a significant activity of high radical attraction. The antimicrobial activity of AuNPs, chrysin and CHR-AuNPs against Staphylococcus aureus and Escherichia coli has also been studied. The results showed an increase in the inhibition of chrysin and AuNPs treated sample, and this activity was more robust in CHR-AuNPs. The cytotoxic effect of CHR-AuNPs on the human breast cancer cell line (AMJ13) was assessed using two methods. The first is the proliferation and growth index, which was determined by determining the inhibitory concentration (IC50), and the results revealed a significant reduction. In a concentration-dependent manner, IC was 125 g ml-1 for both variance and growth (p≤ 0.05). The crystal violet assay was used to perform the second technique. Cell viability was suppressed in a concentration-dependent manner by CHR-AuNPs in AMJ13 cells. (This study has been prepared for the graduate thesis graduation requirement at Ondokuz Mayıs University, Institute of Graduate Studies, Department of Nanoscience and Nanotechnology, Samsun, Turkey.)
Anahtar Kelimeler: Gold nanoparticles, Chrysin, Antioxidant, Antibacterial, Cytotoxicity, AMJ13cells
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